Tobacco plants were treated by removing some of their leaves at different growth stages in a comparative field trial to observe the effect of the treatments on the growth and development of leaves and on the quality of the leaves cured. The results showed that Treatment T3 contributed to the expansion of tobacco leaves, increased the leaf area for photosynthesis and dry matter accumulation of tobacco plant, while Treatment T1 increased the ratio of fresh to dry leaf weight and enhanced plant resistance of tobacco leaves. Treatment T3 showed better chemical composition. Generally, 3 leaves from the bottom of the tobacco plant were not harvested for flue-curing and 2 top leaves were not harvested for flue-curing at the end of harvest stage. This could help improve the quality of tobacco leaves flue-cured.